individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. Small, unbound molecules rotate rapidly in solution (top), while large,. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Page: 1/6. SpectraMax Quant dsDNA Assay Kits. Microplate Reader Shelf. Softmax Pro GxP Software 6. We would like to show you a description here but the site won’t allow us. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. D 1 1. Multimode Reader Comparison Chart. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Instructions on the proper use of the SpectraMax M5 Plate Reader. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. SpectraMax Injector Cartridge with SmartInject™ Technology. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 0 out of 5 $ 5,999. 2. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Microplate Readers include the M3, M4,. Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. . Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Set the excitation at 531 nm and emission at 595 nm. 304 signal level saturation. 2. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Table of Contents. 2. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. . SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The plate reader can measure multiple readouts, including UV and visible absorbance. . Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. . ORDER. 3. Cavicide (Warehouse, Cat # 79300360) 6. When a feature or capability applies to only certain readers, this exception is noted. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. +44-118-944-8000. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Softmax Pro GxP Software 6. Table 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1321N1, a human astrocytoma cell line, expresses. Our absorbance plate readers feature our PathCheck Sensor technology and. you can use a resource. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. e. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. 306 plate air cal fail,low light. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. com A. Monday to Friday 7am - 5pm Pacific Time. 190-1000. type Clear. The excitation and the emission wavelengths on. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. . Add to Cart. com A. Find regional support. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). +1 800-635-5577. 1. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. M5. 6. SpectraMax Microplate Readers. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. $4,499. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. edu) to be added as a trained user in iLab. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. EarlyTox Live Cell Assay (6. Step 1: Open the SoftMax Pro IMAP FP protocol. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Softmax Pro GxP Software 6. Customer Satisfaction is our number one goal All lab equipment in stock. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. . 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. 5. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Weight. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. no. Also refer to this Multimode Reader Comparison Chart. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Settings not required by a reader are indicated by ‘---’. The. Data Acquisition & Analysis Software. Pick the best speed, sensitivity, and price for your needs. Examples: “Press carefully to engage,” “ Do not. Description 1. The following document is intended to demonstrate setup of this instrument. 2. View the Molecular Devices website. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. the IMAP Platform and smoothly transition into high-throughput screening. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Download PDF versions of the SpectraMax M5 User Guides from this page. 3. Recertification is recommended every two years. spr SpectraMax iD5 SpectraTest ABS2 v1. . HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 312 gain calibration failed. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. Third this recommendation. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 1. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Features. . SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. . The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. edu) to be added as a trained user in iLab. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. . Samples were analyzed in eight to sixteen wells per independent. Italic and boldface type are used for emphasis. . Select a rugged, lightweight model designed to be used in the field. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. Fluorescent Polarization: Solid black plates, as above. Select the fluorescence option and read the total fluorescence. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. Figure 2. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The chamber. 7" color, capacitive touch screen. Table of Contents. type Clear. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Consistent results are obtained over time offering workflow flexibility. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. 5. The chamber contains. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Step 3. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 3. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The integration time suggested is 5-10 sec. the IMAP Platform and smoothly transition into high-throughput screening. TR-FRET assay. Table of Contents. Softmax Pro GxP Software 6. . Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The following document is intended to demonstrate setup of this instrument. Step 1: Open the SoftMax Pro IMAP FP protocol. 9000-0756. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. SpectraMax Spectrofluorometers. 4. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. • Tel. 3: The cuvette chamber. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Example: [Drawer]. Catalog Number: M5. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. Add to Cart. KeySpan adapter for SoftMax Pro and SpectraMax readers. 6: bioNova científica, s. In either case. For very concentrated samples, use a minimum2. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. ISM Equipment Classification (Group 1, Class A) This equipment is. edu) to be added as a trained user in iLab. . The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Figure 1. High tolerance for contaminants. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Spectral Range. Introduction. Statistical analyses The statistical analyses were performed using Graph Prism version 7. 2. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Example: [Drawer]. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. SpectraMax M5e Multi-Mode Microplate Reader. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. The plate reader. UV/Vis Photometric Performance:. SoftMax Pro. . For example, microplates containing different samples can be read using the312 gain calibration failed. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. 0 (IBM Corporation, Armonk, NY, USA). Note: In this user guide, all references to SpectraMax Multi-Mode. It may be installed in the top or bottom read detection cartridge transport. There were 12 replicate wells per dilution in both 96-well and 384- well plates. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. 5. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. The following document is intended to demonstrate setup of this instrument. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Dual monochromators provide the ability for. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. 4. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Molecular Devices SpectraMax M5 Multimode Microplate Reader. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 9000-0756. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. spr SpectraMax Plus 384. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. . I have been using the SpectraMax M5 since 2011. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Exhibit with us {{>currencyLabel}} Back. Add to Cart. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. . STAKMAX. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Wavelength Range. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax M5 Multi-Mode Microplate Reader. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 7°C and the bottom to 36. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. view more . 4. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The M5 can read volumes down to 2uL with the uMax Low. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. Molecular Devices. 2. . Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax M5 is a microplate reader that can be used for various applications,. $2,264. Fluorescence Microplate Reader Comparison Chart. acquired on a SpectraMax M5 (Molecular Devices). The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 3. 306 plate air cal. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. ) Click the "OK" button on SoftMax Pro Setup dialog box . Description. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). The chamber. SpectraMax M2 and SpectraMax M2e are controlled by an external computer running SoftMax Pro software which provides integrated instrument control, data display, andQualify the fluorescence performance of the SpectraMax . Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The. D 1. The assay window spanned four orders of magnitude. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Description 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. l. These all-inclusive assay kits are ready to use in assay validation or screening. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). 7 nM EGF, the EC 80 concentration determined from. The SoftMax® Touch Software is installed in. These all-inclusive assay kits are ready to use in assay validation or screening. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . When the correct COM port is selected, the. SpectraMax® Microplate Readers and SoftMax® Pro Software. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. D 1 1. . 4.